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Deficiency of METTL3 restrains the survival of <t>RPMI8226</t> and U266 cells. A/B. RT-qPCR/Western blot measured METTL3 expression in RPMI8226 and U266 cells after transfection; C. CCK-8 assay detected cell proliferation; D. Transwell assay detected cell migration and invasion; E. Flow cytometry detected cell apoptosis; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-NC group; # P < 0.05 vs. the pcDNA-NC group.
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Deficiency of METTL3 restrains the survival of RPMI8226 and U266 cells. A/B. RT-qPCR/Western blot measured METTL3 expression in RPMI8226 and U266 cells after transfection; C. CCK-8 assay detected cell proliferation; D. Transwell assay detected cell migration and invasion; E. Flow cytometry detected cell apoptosis; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-NC group; # P < 0.05 vs. the pcDNA-NC group.

Journal: Journal of Bone Oncology

Article Title: Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression

doi: 10.1016/j.jbo.2025.100695

Figure Lengend Snippet: Deficiency of METTL3 restrains the survival of RPMI8226 and U266 cells. A/B. RT-qPCR/Western blot measured METTL3 expression in RPMI8226 and U266 cells after transfection; C. CCK-8 assay detected cell proliferation; D. Transwell assay detected cell migration and invasion; E. Flow cytometry detected cell apoptosis; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-NC group; # P < 0.05 vs. the pcDNA-NC group.

Article Snippet: The human MM cell lines RPMI8226 and U266 were obtained from ATCC.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Transfection, CCK-8 Assay, Transwell Assay, Migration, Flow Cytometry, Standard Deviation

Downregulation of MALAT1 represses the growth of RPMI8226 and U266 cells. A. RT-qPCR measured MALAT1 expression in RPMI8226 and U266 cells after transfection; B. CCK-8 assay detected cell proliferation; C. Transwell assay detected cell migration and invasion; D. Flow cytometry detected cell apoptosis; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the MALAT1-NC group.

Journal: Journal of Bone Oncology

Article Title: Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression

doi: 10.1016/j.jbo.2025.100695

Figure Lengend Snippet: Downregulation of MALAT1 represses the growth of RPMI8226 and U266 cells. A. RT-qPCR measured MALAT1 expression in RPMI8226 and U266 cells after transfection; B. CCK-8 assay detected cell proliferation; C. Transwell assay detected cell migration and invasion; D. Flow cytometry detected cell apoptosis; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the MALAT1-NC group.

Article Snippet: The human MM cell lines RPMI8226 and U266 were obtained from ATCC.

Techniques: Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, Transwell Assay, Migration, Flow Cytometry, Standard Deviation

Overexpression of MALAT1 reverses the inhibitory effect of METTL3 knockdown on tumor cell growth. A. RT-qPCR measured MALAT1 expression in RPMI8226 and U266 cells after transfection; B. CCK-8 assay detected cell proliferation; C. Transwell assay detected cell migration and invasion; D. Flow cytometry detected cell apoptosis; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-METTL3 + MALAT1-NC group.

Journal: Journal of Bone Oncology

Article Title: Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression

doi: 10.1016/j.jbo.2025.100695

Figure Lengend Snippet: Overexpression of MALAT1 reverses the inhibitory effect of METTL3 knockdown on tumor cell growth. A. RT-qPCR measured MALAT1 expression in RPMI8226 and U266 cells after transfection; B. CCK-8 assay detected cell proliferation; C. Transwell assay detected cell migration and invasion; D. Flow cytometry detected cell apoptosis; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-METTL3 + MALAT1-NC group.

Article Snippet: The human MM cell lines RPMI8226 and U266 were obtained from ATCC.

Techniques: Over Expression, Knockdown, Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, Transwell Assay, Migration, Flow Cytometry, Standard Deviation